A sensitive, precise and selective ultra-high performance liquid chromatography\nmethod coupled with triple-quadrupole mass spectrometry was developed and validated\nfor the determination of trace amounts of sinomenine (ng/mL) in minute volumes of\nhuman plasma. Fifty microliter plasma samples were precipitated using methanol to extract\nsinomenine. Separation was carried out on a C18 column with a water and acetonitrile\nmobile phase gradient with formic acid as an additive. The mass spectrometry data were\nobtained in the positive ion mode, and the transition of multiple reactions was monitored at\nm/z 330.2181.0 for sinomenine quantification. The working assay range for sinomenine\nwas linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL.\nThe precision and accuracy of the method was less than 15% in intra-day and inter-day\nexperiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal\ndelivery of the Zhengqing Fengtongning Injection. The results showed that some samples\ncontained sinomenine within the concentration range 0.4131ââ?¬â??4.407 ng/mL.
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